A stevia cultivar, designated '16228013', is disclosed. Further embodiments relate to the plant parts of stevia cultivar '16228013', to the plants of stevia '16228013' and to methods for producing a stevia plant produced by crossing the cultivar '16228013' with itself or another stevia variety, including methods using marker assisted breeding. Further embodiments relate to hybrid stevia seeds and plants produced by crossing the cultivar '16228013' with another stevia cultivar. Six highly polymorphic SNPs loci and the corresponding genomic sequences used to identify plant variety '16228013'derived plant materials are also disclosed.