Neel S. Joshi,Peter Q. Nguyen,Anna Duraj-Thatte,Pichet Praveschotinunt
申请号：
US15564619
公开号：
US20180258435A1
申请日:
2016.04.06
申请国别(地区):
US
年份:
2018
代理人:
摘要：
Embodiments of the present disclosure are directed to methods of genetically modifying bacteria to create amyloid-based materials, such as biofilms created by amyloid fibers, having nonnative functional polypeptides expressed thereon and connected thereto by a linker domain optimized for functioning of the non-native functional polypeptides. According to one aspect, the linker domain is optimized for functioning of a CsgA protein as is it assembled into an amyloid state and for functioning of the functional polypeptide. Exemplary biofilms may include living bacterial cells, non-living bacterial cells or combinations of living bacterial cells and non-living bacterial cells. Methods of making biofilms having non-native functional polypeptides attached thereto are provided.
