Disclosed herein are recombinant polypeptides derived from FBP1 and FBP2. Also disclosed herein are recombinant expression vectors and recombinant host cells for producing the aforesaid recombinant polypeptides. The recombinant polypeptides are proven to be useful and effective in producing a picornavirus with a type I internal ribosome entry site (IRES), so as to facilitate the preparation of a viral vaccine.本發明揭示衍生自FBP1與FBP2的重組型多肽。本發明亦揭示用於生成前述的重組型多肽的重組型表現載體以及重組型宿主細胞。該等重組型多肽被證實在生成一具有一第I型內部核醣體進入位址(IRES)之微小核醣核酸病毒上是有用且有效的,藉此有助於一病毒疫苗的製備。