PURPOSE: A composition for preventing rice bacterial blight is provided to suppress rice bacterial blight which causes the biggest damage to rice plants, and to minimize damage of a rice growing region. CONSTITUTION: A composition for preventing rice bacterial blight contains dunaimycin D3S of chemical formula I. Dunaimycin D3S is derived from Streptomyces bottropensis MJM3389. The rice bacterial blight is a plant disease caused by Xanthomonas genus bacteria. A method for preparing dunaimycin D3S comprises the steps of: culturing Streptomyces bottropensis MJM3389; extracting the culture with an organic solvent; performing chromatography of the extract and obtaining an active fraction; and purifying the active fraction.본 발명은 스트렙토마이세스 보트로펜시스 MJM3389(Streptomycesbottropensis MJM3389) 균주로부터 생산되는 두나이마이신 D3S(Dunaimycin D3S)를 포함하는 벼흰잎마름병 방제용 조성물에 관한 것으로, 본 발명에 따른 벼흰잎마름병 방제용 조성물은 세계 3대 식물 중 하나인 벼의 가장 큰 피해를 야기하는 벼흰잎마름병을 억제하여 벼 재배 지역의 피해를 최소화할 수 있다.