A particle of microbial fertilizer is provided, and includes components mixed well and described below: a culture solution containing probiotic endospores, an aqueous solution of polyol, a bio-degradable filler material, a bio-degradable polymer, and a nutritive substance. The particle of microbial fertilizer extends storage time of microbial fertilizer and maintains viability of probiotics, and thus effects of microbial agent are successfully achieved.一種微生物肥料顆粒,包括經均勻混合的以下組份:一益生菌內孢子培養液、一多元醇水溶液、一生物可分解填充材料、一生物可分解聚合物以及一營養物質。微生物肥料顆粒能大幅增加微生物肥料的保存期限,以及維持益生菌的存活率,進而充分發揮微生物製劑的功效。S100、S110‧‧‧步驟