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METHODS FOR CONJUGATING NUCLEIC ACIDS WITH SMALL MOLECULES
专利权人:
Tzu-Pin Wang
发明人:
Tzu-Pin Wang,Yi-Jhang Ciou
申请号:
US13188989
公开号:
US20120177573A1
申请日:
2011.07.22
申请国别(地区):
US
年份:
2012
代理人:
摘要:
A method for conjugating a nucleic acid with a molecule is provided. The method includes steps of (a) reacting the nucleic acid having a 5′-monophosphate with an activating agent in a first buffer to form a solution (b) mixing an alcohol with the solution formed in the step (a) to obtain an intermediate and (c) dissolving the intermediate in a second buffer containing an ethylenediaminetetraacetic acid (EDTA) and adding a nucleophile thereinto to react the intermediate with the nucleophile.
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