A urine collection device has a chamber assembly, which includes a layer of wicking material and porous material. The porous material is configured to form a chamber in which urine can be collected for transport; and the wicking material is disposed about the porous material. The chamber has a port for receiving a tube so that urine collected within the chamber can be transported from the chamber by being drawn from the chamber when a partial vacuum is applied within the chamber via the received tube. The chamber assembly is so dimensioned and configured that opposing portions of the assembly are sufficiently adjacent as to define an opening through which the head of a penis can be inserted. A layer of impermeable material is so attached to the chamber assembly as to cover one side of the opening and thereby provide a receptacle for receiving the head of an inserted penis.