REUTELINGSPERGER, CHRISTIAAN PETER MARIA,MOONEN, PETER,VERMAIRE, AD
申请号：
NZ56907806
公开号：
NZ569078A
申请日:
2006.12.12
申请国别(地区):
NZ
年份:
2012
代理人:
摘要：
Disclosed is an annexin variant corresponding to annexin A5 having the ammo acid sequence of SEQ ID NO: 1: a) that binds to at least one phospholipid b) that is not internalized into a cell, wherein one or more amino acids selected from polar amino acids Glu, GIn, Asp, Asn, Arg and Lys in the helices IA, ID, IIA, IID, IIIC, IIID and IVE and in the stretches connecting helices IC and ID, IIE and IIIA, IIIC and IIID, IIID and IIIE, and IVA and IVB are replaced by non-polar amino acids c) that is bound to a recognizable compound through a cysteine residue of the annexin variant. Also disclosed is an annexin variant wherein the annexin comprises SEQ ID NO: 1 for annexin A5 or the corresponding sequences for other annexins, wherein one or more amino acids selected from Glu, GIn, Asp, Asn, Arg, Lys and His at positions 16-29, 59-74, 88-102, 135-145, 156-169, 202-231, 259-266 and 305-317, or the corresponding sequences for other annexins, are replaced by Gly, Ala, Val, lIe, Leu, Ser, Thr, Met, Pro, Phe, or Tyr, and wherein one of the amino acids at positions 1-19, 24, 28,46-64, 86-89, 118-135, 149-150, 157-170, 203-219, 245-248 and 280-294 has been replaced by a cysteine residue, which is optionally substituted.
