The purpose of the present invention is to provide: a pharmaceutical composition comprising a human serum enzyme-treated product that is useful for treating or preventing diseases such as cancer and infections; and a method for manufacturing said pharmaceutical composition. The present invention pertains to: a method for manufacturing a pharmaceutical composition comprising a human serum enzyme-treated product, said manufacturing method including a step for bringing human serum into contact with β-galactosidase; and a pharmaceutical composition comprising a human serum enzyme-treated product obtained according to said manufacturing method.