A physiological detection method includes following steps. A detection portion of a human body is detected for obtaining a detection signal. Then, the detection signal is processed for outputting a digital physiological signal. The digital physiological signal is received for obtaining a first information and a second information of feature points thereof. Then, a ratio of the second information and the first information is calculated for obtaining a physiological condition index. The digital physiological signal has a plurality of waves generated according to a time sequence. The feature points of the digital physiological signal include a wave crest point and a foot point located at a forepart of the rising edge of the wave. In addition, a physiological detection device is also mentioned.一種生理檢測方法包括以下步驟:偵測人體的被測部位,以獲取感測訊號。接著,處理感測訊號,以輸出數位化生理訊號。接收數位化生理訊號,以計算獲取數位化生理訊號的特徵點的第一及第二資訊,並且再計算第二資訊與第一資訊的比值,以獲取生理狀態指數。數位化生理訊號包括依時序產生的多個脈波,並且數位化生理訊號的特徵點包括脈波的波峰點及位於脈波的上升緣前端的起始點。此外,一種生理檢測裝置亦被提及。S201~S203‧‧‧步驟