Methods are provided for preparing liquid PRF and using the liquid PRF as a drug-delivery carrier system for other regenerative biomaterials and biomolecules. This carrier system provides more effective regenerative strategies for improved tissue wound healing, treatment, and regeneration in the body by supplying additional autologous growth factors into commonly-utilized biomaterials improving the host-tissue response to such therapies. The method of preparing an isolated serum fraction of liquid PRF comprises spinning a whole blood sample by centrifugation to separate erythrocytes； separating a sample of liquid PRF from the whole blood sample without the addition of an additive； spinning the sample of liquid PRF instantly by centrifugation carried out at 20 to 950 G for 2 to 8 minutes； and collecting an isolated serum fraction containing the liquid PRF using an application device.
