The present invention relates to the field of biomedicine, and discloses a class of selenium-containing inhibitors that bind to the allosteric site of glutaminase, and/or inhibit glutamate dehydrogenase, which has a long carbon chain or similar derivative structure linked with the N atom of benzoisoselenazole 3-ketone. The advantages of this invention are that compounds have novel binding activity with glutaminase and potent inhibitory activity of glutaminase. Moreover, some novel dual KGA/GDH inhibitors demonstrated good in vivo efficacy in inhibiting the tumor growth in mice by blocking glutaminolysis. At the meantime, compounds of this invention play an important part in the protection of survival, growth and differentiation of nerve cells under the condition of hypoxia-ischemia.
