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Aldolases, nucleic acids encoding them and methods for making and using them
专利权人:
BASF ENZYMES LLC
发明人:
David P. Weiner,Lishan Zhao,Peter Luginbuhl,Ellen Burke,Toby H. Richardson,Paula M. Hicks
申请号:
US13966880
公开号:
US09290755B2
申请日:
2013.08.14
申请国别(地区):
US
年份:
2016
代理人:
摘要:
This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.
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