A blood isolation and extraction method includes: providing a predetermined amount of blood； utilizing a platelet filter unit to filter the predetermined amount of blood to generate a filtered blood； utilizing a plasma separation unit to divide the filtered blood into a plasma layer and a blood cell layer for separating blood cells from blood plasma； and extracting the blood plasma from the plasma layer and the blood cells from the blood cell layer. In another embodiment, the blood isolation and extraction method further includes: providing a platelet-washing unit to wash the platelet filter unit with a solution to produce a platelet solution； and mixing the platelet solution with the blood plasma to produce a platelet and plasma mixed solution.
