The present invention relates to pharmaceutical compositions comprising macrophage activating factor (MAF) and method of producing same particularly to MAF compositions essentially devoid of glycosidase enzymes. The compositions of the present invention and pharmaceutical compositions comprising same are particularly suitable for intravenous administration. Thus according to one aspect the present invention provides a composition comprising Gc protein derived macrophage activating factor (GcMAF) wherein the composition is essentially devoid of glycosidase enzymes.
