The invention relates to derivatives of glucagon analogues comprising the substitutions Imp1 and His3, a substituent having three to ten negatively charged moieties covalently attached to a side chain of a lysine as well as intermediates and compositions thereof and their use in medicine.本發明係關於升糖素類似物之衍生物,其包含取代Imp1及His3、具有三至十個帶負電部分之共價連接於離胺酸之側鏈的取代基;以及其中間物及組成物及其在醫學中之用途。