A short-chain peptide capable of controlling spermatozoa fertilization performance is disposed in an in vitro culture medium of spermatozoa and oocytes to increase the success rate of in vitro fertilization. The short-chain peptide comprises an amino acid sequence (SEQ ID NO:1) H-Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-X-NH2, where X denotes Tyr or Phe, and the sequence ends with an amide. The short-chain peptide is conducive to adjustment of the concentration of free calcium ions inside the cells of the spermatozoa and thus enhancement of the success rate of in vitro fertilization.本發明係提供一種調控精子授精能力之短鏈胜肽,該短鏈胜肽係添加於包含精子及卵子的體外培養液,以提高體外人工授精之成功率,其特徵在於:該短鏈胜肽係包含一氨基酸序列(SEQ ID NO:1) H-Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-X-NH2,其中X係為Tyr或Phe,而序列末端為醯胺(amide)。藉由含有該短鏈胜肽來調節精子細胞內自由態鈣離子之濃度,而完成提高體外人工授精之成功率。無
