Method for regulating cancer stem cell growth by inhibiting phosphorylation of 120th threonine residue of TSPYL5 protein, a composition containing the peptide sequence functioning to inhibit the phosphorylation and a use thereof
In Gyu Kim,Seo Yeon Kim,Jei Ha Lee,Soo Im Choi,Min Sik Kim,Jung Yul Kim,Byungchul Shin,Uhee Jung
申请号：
US15774208
公开号：
US10117903B1
申请日:
2017.06.15
申请国别(地区):
US
年份:
2018
代理人:
摘要：
The present invention relates to a peptide suppressing the phosphorylation of threonine (T120), the 120th residue of TSPYL5 (testis-specific Y-like protein 5), which is specifically as follows. The present inventors constructed T120D, the mutant of the 120th residue threonine (T120) of TSPYL5, and T120A-TSPYL5 gene and then transfected cells with them in order to investigate the effect of phosphorylation on T120 residue. As a result, wild-type TSPYL5 and T120D moved into nucleus and stayed there. But in the case of T120A-TSPYL5, TSPYL5 did not move into nucleus and instead it was expressed only in cytoplasm. The protein could not bind to AKT, either. Instead, ubiquitination of TSPYL5 was increased but SUMOylation was inhibited. Also, the expressions of ALDH1-A1, -A3, CD44 gene and protein were reduced, and thereby the growth and metastasis of lung cancer cells were suppressed and sphere formation was reduced. Based on the observation above, the inventors constructed the peptide composed of the amino acid sequences represented by SEQ. ID. NO: 43 or NO: 44 that could inhibit phosphorylation of the 120th residue threonine of TSPYL5. The said peptide can be effectively used as a composition for the inhibition of cancer cell growth, metastasis, and cancer stem cell growth.
