A gas transfer system including a housing, a pressurized gas canister held by the housing, a first passageway in fluid communication with the pressurized gas canister and configured to supply pressurized pre-sterilization gas from the pressurized gas canister to a fluid flow component, a gas discharge canister held by the housing and a second passageway in fluid communication with the gas discharge canister and configured to supply post soak gas from the fluid flow component to the gas discharge canister. A humidification system or device is included for the sterile humidification of sterilizing gas. Additional related devices, systems and methods are provided.
