A stem cell formulation for anti-aging repair, said stem cell formulation comprising umbilical cord, placental or amniotic mesenchymal stem cells, a platelet lysate, human serum proteins, a NaCl solution, fasudil, doxycycline, a polymeric stabilizer, a cell anti-freeze agent and a humectant. A method for preparing a stem cell formulation, comprising the following steps: S1-S10, thus obtaining a stem cell formulation. This invention guarantees cell viability during the period of validity, breaking through the technical bottleneck of mesenchymal stem cells for clinical treatment； and this formulation can be applied to the affected part directly after the container thereof being opened, without requiring cumbersome operations, and can achieve the purposes of rapidly repairing damaged skin, improving cell proliferation viability, anti-aging, and repairing damaged skin. The preparation method is simple and easy to operate, is convenient for mass production, and the formulation obtained according to the present method is stable, and can exert a therapeutic effect continuously for a long time. This invention can be used in the fields of skin cosmetics, medical plastic and trauma repair.L'invention concerne une formulation de cellules souches pour une réparation anti-vieillissement, ladite formulation de cellules souches comprenant des cellules souches mésenchymateuses de cordon ombilical, placentaire ou amniotique, un lysat de plaquettes, des protéines sériques humaines, une solution de NaCl, du fasudil, de la doxycycline, un stabilisant polymérique, un agent anti-gel pour cellules, et un humectant. L'invention concerne également un procédé de préparation d'une formulation de cellules souches, comprenant les étapes suivantes S1 à S10, permettant d'obtenir une formulation de cellules souches. La présente invention garantit la viabilité cellulaire pendant la période de validité, résolvant le problème technique des cellules souches mésenchymateuses destinées à un traitement c
