A multilayer film which exhibits excellent heat resistance and can keep flexibility and transparency even after high -temperature sterilization and which little sticks to a cutter in sealing the peripheral area or cutting away unnecessary parts. The multilayer film comprises the first layer (outer layer) (1) which is made of, e.g., a mixture comprising 55 to 85wt% of a linear polyethylene (PE-L) having a DSC melting point of 120 to 125°C and a density of 0.930 to 0.937g/cm3 and 15 to 45wt% of a propylene homopolymer having a DSC melting point of 160 to 165°C and the second to fifth layers (inner layers) (2) to (5) which are each made of PE-L. The second and fourth layers (2) and (4) have DSC melting points of 120 to 125°C and densities of 0.910 to 0.920g/cm3, while the third layer (3) has a DSC melting point of 120 to 125°C and a density of 0.930 to 0.937g/cm3. Medical fluid bags can be made by using the multilayer film.