Provided herein are methods of co-culturing by quorum sensing, bacterial strains useful in co-culture systems and methods, co-culture systems, and pharmaceutical compositions, drug delivery systems, and methods of treating disease related thereto. In some embodiments, the co-lysis systems are provided that operate in an orthogonal or essentially orthogonal manner. In some embodiments, methods of maintaining a co-culture by quorum sensing are provided that include: co-culturing at least two bacterial strains at certain ratios during a period of time； wherein: at least one of the at least two bacterial strains has a growth advantage compared to at least one other bacterial strain； at least one of the at least two bacterial strains comprises a lysis plasmid and an activator plasmid. Also provided herein are pharmaceutical composition that include any of the bacterial strains described herein.L'invention concerne des souches bactériennes, des procédés de culture de cellules bactériennes utilisant une lyse régulée par quorum synthétique, et leurs utilisations.
