A liquid medicine preparation kit that allows substantially aseptic operationand ensures easy handling, being free from the danger of scattering of aerosolto environment and liquid leakage in the form of droplet, etc. at the time of liquidmedicine preparation. There is provided a liquid medicine preparation kitincluding a prefilled syringe and a transfer pour tool, characterized in thatthe prefilled syringe is fitted with a sealing member for sealing of the distalend thereof, the sealing member not detachable from the distal end, and that thetransfer pour tool is fitted with a one-way valve capable of irreversible emissionof only the gas within the system and a filter disposed so as to be adjacent to theside of second communication channel from the one-way valve.