The invention relates to a method for improving infection rate of hosts of parasites of cordyceps sinensis. By the method, body surfaces of larvas of the hosts of the parasites of the cordyceps sinensis are illuminated by laser light, tiny wounds are generated, hirsutella sinensis bacteria can enter body cavities of the larvas of the hosts of the parasites easily, and fruiting bodies of the cordyceps sinensis can be generated. The index components of the fruiting bodies, which are obtained by the artificial culture method, of the cordyceps sinensis are the same with those of fruiting bodies of wild cordyceps sinensis. By the method, the infection rate and the survival rate of the larvas can be improved greatly and are not limited by degree of proficiency of operators, the larvas of the hosts of the parasites of the cordyceps sinensis can be infected quickly and widely, the problem that the infection rate and the survival rate of the larvas, which are infected artificially in the prior art, of the hosts of the parasites of the cordyceps sinensis are low is solved, and the method can be used for artificial large-scale breeding and production of the cordyceps sinensis and has an important popularization role in artificial cultivation of the cordyceps sinensis.