Systems, methods, and ultrasound transceivers equipped and configured to execute harmonic analysis and extract harmonic information related to a targeted organ of a subject are described. The methods utilize neural network algorithms to establish improved segmentation accuracy of the targeted organ or structures within a region-of-interest. The neural network algorithms, refined for detection of the bladder and to ascertain the presence or absence of a uterus, is optimally applied to better segment and thus confer the capability to optimize measurement of bladder geometry, area, and volumes.