HYBRIDOMA CELL LINE PRODUCING MONOCLONAL ANTIBODIES AGAINST THE FOOT-AND-MOUTH DISEASE VIRUS, THE MONOCLONAL ANTIBODIES THEREFROM, AND REAGENT AND ELISA KIT COMPRISING THE SAME
ANIMAL HEALTH RESEARCH INSTITUTE; COUNCIL OF AGRICULTURE
发明人:
CHEN, TSU HAN,陈姿菡,陳姿菡,LEE, FAN,李璠,李璠,PAN, CHU HSIANG,潘居祥,潘居祥,JONG, MING HWA,锺明华,鍾明華
申请号：
TW098123672
公开号：
TWI392735B
申请日:
2009.07.14
申请国别(地区):
TW
年份:
2013
代理人:
摘要：
The present invention provides a hybridoma cell line producing monoclonal antibodies against the foot-and-mouth disease virus, the monoclonal antibodies therefrom, reagent and kit for ELISA, and immune detection method. The hybridoma cell line is produced from a parental cell and a myloma cell line and has the same characteristic as the the cell line whose deposition number is BRBC 960397. The parental cell the spelenocytes isolated from spleen of the mouse that is immunized by an antigen that is derived from the 3ABC non-structure protein of FMDV. The antigen used here is expressed by a prokaryotic cell. The monoclonal antibody produced by the hybridoma can specifically recognize the 3ABC polypeptide and does not cross-react with the antiserum of swine vesicular disease virus.本發明係提供一種融合瘤細胞株、其單株抗體、包含前述單株抗體之免疫檢測試劑及套組,以及免疫檢測方法。融合瘤細胞株係由一親代細胞與一骨髓瘤細胞融合而製成,其特徵如寄存編號為BRBC 960397之細胞株所示,該親代細胞係由一原核細胞所表現之一口蹄疫病毒之一由3ABC基因片段所編碼之非結構蛋白作為抗原免疫至一小鼠,並將該小鼠體內之一脾臟細胞取出而製成,且該融合瘤細胞株所生產之單株抗體能夠專一性辨識一口蹄疫病毒之該3ABC胜肽,且不會與豬水疱病抗血清產生非特異性反應。10‧‧‧固相載體11‧‧‧單株抗體12‧‧‧樣本抗原13‧‧‧待測檢體14‧‧‧偵測抗體15‧‧‧訊號產生物質16‧‧‧受質
