The invention provides a method and system for non-invasive analysis, especially suitable for determining attributes of targets, such as measurement of layer thickness of tissue, or the concentration of glucose within human tissue. The invention includes an optical source and an optical processing system that provides probe and reference radiation applies the probe beam to the target to be analyzed re-combines the probe and reference beams interferometrically, to generate one or more concurrent interferometric signals that are detected and processed. The location within the target to which an interference signal relate is aligned and registered with respect to the target is by means of a field of light based imaging device. The invention is not limited to human tissue in vivo, nor to in vivo non-invasive analysis.
