The present invention relates to the field of preservation of microorganisms, and in particular provides a 2-compartment system comprising a first compartment comprising microcapsules with microorganisms in a non-aqueous composition in the core of said microcapsules, and a second aqueous organic acid-containing compartment. It further provides methods for preserving microorganisms, on the basis of such systems, as well as uses of such systems for preserving microorganisms.