Viki Bowman Vance,Lewis Howard Bowman,Allison Mallory
申请号:
US13282680
公开号:
US09822376B2
申请日:
2011.10.27
申请国别(地区):
US
年份:
2017
代理人:
摘要:
Compositions and methods for modulating nucleotide sequence expression, particularly for modulating gene expression in plants, are provided. The compositions comprise precursor RNA constructs for the expression of an RNA precursor. The precursor RNA construct comprises a promoter that is expressed in a plant cell driving the expression of a precursor RNA having a microRNA. The miRNA is complementary or partially complementary to a portion of a target gene or nucleotide sequence and function to modulate expression of the target sequence or gene. In this manner, the RNA precursor construct can be designed to modulate expression of any nucleotide sequence of interest, either an endogenous plant gene or alternatively a transgene. Transformed plants, tissues, cells and seeds are also provided.