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Novel Cryopreservation Bags and method of use thereof for Closed System, High Capacity Cell-Banking
专利权人:
Merial Inc.
发明人:
Coupier Hervé François
申请号:
US201715840701
公开号:
US2018100135(A1)
申请日:
2017.12.13
申请国别(地区):
美国
年份:
2018
代理人:
摘要:
The disclosure provides a cell freezing and storage bag assembly and a method for using the assembly in banking eukaryotic cells for later seed train expansion. The bag is constructed principally of fluorinated ethylene propylene (FEP) fabric, and is designed to be filled such that the cell suspension has a very thin cross-section. The bag design includes at least an inlet conduit and an outlet or inoculation conduit, which can be sterilely welded to the source of the eukaryotic cells. The use of at least two sterile-weldable conduits allows for “closed system” filling of the bags, which significantly reduces the risk of contamination relative to other cell-banking methods. The bag also include a sleeve, which can be thermo-welded to form an enclosure, which protects the inlet and outlet conduits against contamination and mechanical damage during freezing, storage and subsequent thawing. In the method, once each bag is filled, its corresponding inlet conduit is sealed, and both the inlet and outlet condui
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