The invention discloses a method for producing a compound (5-epi-4 "-N- (L-isocetyl) apramycin) and 5-epipromycin, and an intermediate used in the method. The method of the present invention is useful from the viewpoint that the compound (5-Table-4 "-N- (L-isocetyl) apramycin) can be produced efficiently and safely.本發明揭示一種化合物(5-表-4”-N-(L-異鯨蠟基)安普黴素)及5-表安普黴素的製造方法,以及使用於該方法的中間體。本發明之方法由可有效率且安全地製造出化合物(5-表-4”-N-(L-異鯨蠟基)安普黴素)之觀點來看為有用。