PROBLEM TO BE SOLVED: To provide a nano thin film transfer sheet comprising a nano thin film layer formed with good work efficiency, and a method of producing a nano thin film transfer sheet capable of shortening the time for the formation of the nano thin film layer and having excellent transferability, and a method of transferring the nano thin film layer of the nano thin film transfer sheet to an adherend.SOLUTION: A nano thin film transfer sheet comprises a permeable base material which is a base material allowing a solvent to permeate or pass through it, and a nano thin film layer comprising chitosan including vegetable chitosan or β-glucan chitosan.COPYRIGHT: (C)2015,JPO&INPIT