#$%^&*AU2014203338B220180802.pdf#####Abstract The invention describes a method for preparing tissue for medical applications, in particular tissue for use for an artificial heart valve, wherein the method has the steps, at 5 the least, of: - decellularizing the tissue by means of a detergent and subsequently - cross-linking the collagen fibers of the tissue by means of a suitable crosslinking agent. At least one lipopeptide, such as surfactin, for example, is used as the detergent for 10 decellularization. FIG. 1 5493217_1 (GHMatters) P97349.AU 17/06/14