Process for high efficiency refolding of recombinant proteins

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专利权人:

发明人:

Sanjay Sonar,Archana Krishnan,Nikhil Ghade,Faiza Shaikh

申请号：

IN512/MUM/2013

公开号：

IN2013MU00512A

申请日:

2013.02.22

申请国别(地区):

IN

年份:

2014

代理人:

摘要：

A process of refolding a recombinant protein from inclusion bodies (IBs) formed inside a host cell, is provided. The process includes homogenising a wet cells slurry to obtain a cell lysate incubating the cell lysate with a reducing agent to obtain a reduced cell lysate isolating reduced IBs from the reduced cell lysate to obtain isolated IBs solubilising the isolated IBs with a denaturing agent to obtain solubilised IBs and subjecting the solubilised IBs to a unit process of refolding to obtain a refolded recombinant protein.

来源网站：

中国工程科技知识中心

来源网址：

http://www.ckcest.cn/home/