Hryz Biotech Co.;Shenzhen Institutes of Advanced Technology
发明人:
Yifan MA,Xiangjun ZHOU,Shang CHEN,Lintao CAI,Ce WANG,Peng LIU
申请号：
US15312976
公开号：
US20170296639A1
申请日:
2015.05.20
申请国别(地区):
US
年份:
2017
代理人:
摘要：
The present invention provides a method for preparing dendritic cell loaded with antigen, the method comprising the steps of adding serum-free cell culture medium containing granulocyte-macrophage colony-stimulating factor (GM-CSF) and inter-leukin (IL)-4 into mononuclear cells, culturing in an incubator at 37° C. under 5% carbon dioxide for 5 days, adding target antigen wrapped cationic liposome and culturing for 8-24 hours to obtain target antigen loaded dendritic cell.
