The present invention relates to new cell (for example, liver cell, etc.) composition and its preparation and application. Particularly, the present invention relates to the products for handling these cells to enable them to the method that repeated freezing saves and thaws while keeping basic viability. The invention further relates to the repeatedly products of freezen protective and the cell of defrosting (for example, liver cell).本發明涉及新的細胞(例如,肝細胞,等等)組合物,及其製備和使用方法。特別地,本發明涉及處理這些細胞的製品以使它們能够重複冷凍保存和解凍、同時保持基本的存活力的方法。本發明還涉及已經重複地冷凍保存和解凍的細胞(例如,肝細胞)的製品。