An eye detection method for detecting a degree of eye opening and closure of a testee includes detecting a location of an eye pupil, a location of an inner eye corner and a location of an outer eye corner of an eye of the testee; calculating an eye width according to the location of the inner eye corner and the location of the outer eye corner; multiplying the eye width by a specific ratio to obtain an eye height; generating a region of interest with a center on the location of the eye pupil, a length equal to the eye width, and a width equal to the eye height; and determining a ratio of the eye occupying a detection area in the region of interest, and determining the degree of eye opening and closure of the testee accordingly.