The present invention relates to a three-dimensional cell culture systems can be used for a variety of different cells (human mesenchymal stem cells, mankinds fibr tissue mother, keratinocyte, external fibrocyte differentiated form mescenchymal stem cell and external keratinocyte differentiation form mescenchymal stem cell) of culture external continuous period. In the present invention, on the frame or matrix pre-established made of chitosan and gelatin, mescenchymal stem cell (MSC) is originated from marrow or control cable is inoculated with and is grown. The organization construction of exploitation includes unit, growth factor, cytokines and other regulatory factors secreted by unit. The organization construction made of bioengineering of exploitation provides the bracket for wound healing, growth factor and required cell factor. The organization construction of exploitation is useful to the treatment of burn wound, the diabetic foot ulcer wound and type of chronic skin wound. It facilitates treatment and the regeneration of skin tissue.
