ДЗЕ ТРАСТИЗ ОФ ДЗЕ ЮНИВЕРСИТИ ОФ ПЕНСИЛЬВАНИЯ (US)
发明人:
ЧЕРНЕЦКИ Брайан Дж. (US),КОЛДОВСКИ Урсула (умер),СЮЙ Шувэнь (US),КОСКИ Гари К. (US)
申请号：
RU2012143745/10
公开号：
RU2012143745A
申请日:
2011.03.15
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. A method for obtaining antigen-loaded, activated dendritic cells (DC) for use in immunotherapy comprising: loading at least one antigen DC DC activation of at least one agonist of TLR cryopreservation DC irazmorazhivanie DC DC which produces an effective amount of at least one cytokine to create T-cell otveta.2. A method according to Claim. 1, wherein the antigen is a tumor antigen.3. A method according to Claim. 1, wherein the antigen is a microbial antigen.4. A method according to Claim. 1 wherein the TLR agonist is LPS.5. A method according to Claim. 1, wherein the cryopreservation includes DC freezing at about -70 ° C or nizhe.6. A method according to Claim. 1, wherein the degree of recovery and viability after thawing DC than or equal to about 70% .7. A method according to Claim. 1, wherein the degree of recovery and viability after thawing DC than or equal to about 80% .8. A method according to Claim. 1, wherein the DC kriosohranyayut for at least about one nedeli.9. A method according to Claim. 1, wherein the cytokine is IL12.10. A method according to Claim. 1, wherein the DC exhibit killer cell function, thereby capable of lysing DC misheni.11-cancerous cells. The method of activation of immune response in a mammal, the method comprising administering to a pre-cryopreservation composition comprising an antigen-loaded, activated DC, to a mammal in need thereof, wherein the antigen loaded DC and activated before kriosohraneniem.12. The method of claim. 11 wherein the antigen is a tumor antigen.13. The method of claim. 11 wherein the antigen is a microbial antigen.14. The method of claim. 11 wherein1. Способ получения нагруженных антигеном, активированных дендритных клеток (DC) для применения в иммунотерапии, включающий:нагрузку по меньшей мере одним антигеном DCактивацию DC по меньшей мере одним агонистом TLRкриосохранение DC иразмораживание DCгде DC продуцирует эффективное количество по меньшей мере одного цитокина для создания T-клеточного ответа.2. Спосо
