The present invention relates to a production device and a production method for dialysate using the reverse injection of RO water. The production method for the dialysate comprises the following steps: supplying the RO water from the lower side of a cartridge containing sodium hydrogen carbonate powder which is a raw material for producing dialysate, to the upper side making the powder be firstly mixed by the pressure of the RO water and move upward making some of the undissolved powder be fallen to both sides of the cartridge for secondly mixing the powder to obtain dialysate and discharging the dialysate through the upper side of the cartridge for storing. The present invention can completely dissolve the powder without the remaining powder on both upper and lower edges inside the cartridge, and can produce dialysate with the conductivity necessary for dialysis.COPYRIGHT KIPO 2014[Reference numerals] (AA) Dissolve powder? (BB,FF) No (CC,DD) Yes (EE) Satisfy conductivity? (S310) Place a cartridge between a first needle unit and a second needle unit (S320) Punch an inflow protrusion cap and an outflow protrusion cap on the upper part and the lower part of the cartridge (S330) Supply RO water to the inflow protrusion cap under the cartridge (S340) Mix and dissolve the RO water and powder (S350,S370) Store dialysate (S360) Re-dissolve the undissolved powder (S380) Drain본 발명은 RO수의 역투입을 이용한 투석액 제조장치 및 방법으로서, 투석액 제조의 원료인 파우더 형태의 탄산수소나트륨이 저장된 카트리지의 하부로부터 상부를 향하여 RO수를 공급하고, 파우더가 상기 RO수의 압력에 의해 1차적으로 섞이면서 위로 올라갔다가 녹지 않은 파우더의 일부가 카트리지의 양측면으로 떨어지면서 2차적으로 더 믹싱되게 투석액을 제조한 후 카트리지의 상부로 배출시켜 저장함으로써, 카트리지 내부의 상하 양측 가장자리에 파우더가 잔류되지 않게 완전히 녹일 수 있게 함과 동시에 투석에 필요한 전도도를 갖는 투석액을 제조할 수 있다.