Federalnoe gosudarstvennoe byudzhetnoe obrazovatelnoe uchrezhdenie vysshego obrazovaniya "Moskovskij gosudarstvennyj universitet imeni M.V. Lomonosova" (MGU)
发明人:
Arkhipova Anastasiya Yurevna (RU),Архипова Анастасия Юрьевна (RU),Ramonova Alla Alikovna (RU),Рамонова Алла Аликовна (RU),Mojsenovich Mikhail Mikhajlovich (RU),Мойсенович Михаил Михайлович (RU),Karach
申请号：
RU2017147018
公开号：
RU0002692578C1
申请日:
2017.12.29
申请国别(地区):
RU
年份:
2019
代理人:
摘要：
FIELD: medicine.SUBSTANCE: implant for bone tissue regeneration consists of composite microparticles characterized by porous structure with pore size from 10 to 85 mcm, content of silk fibroin from 65 to 75 wt. %, content of gelatine from 25 to 35 wt. %, as well as Young's modulus of compression in dehydrated state 83 ± 1 MPa, in wet - 590 ± 60 kPa. Method of implant production includes the following stages: preparing an aqueous solution of fibroin, preparing an aqueous solution of gelatine, forming a composite scaffold, cryomilling the scaffold obtained at the step of forming a composite scaffold, and deposition of composite microparticles obtained at step of cryophasing scaffold. Preparation of water solution of silk fibroin is performed by dissolving fibroin in amount of 100–150 mg/ml in CaCl2 mixture: C2H5OH: H2O in molar ratio of components of mixture of 1:2:8 for 5–7 hours while heating to 70 °C±5 °C and subsequent dialysis against water, centrifugation at 13,400 g for 10 minutes and bringing the obtained solution with water to concentration of 20–30 mg/ml. Preparation of aqueous solution of gelatine is carried out by dissolution of dry gelatine in water at rate of 20–30 mg/ml. Composite scaffold formation is performed by freezing for 6–8 days at temperature - (18–25) °C a mixture of solutions obtained at the steps of preparing an aqueous solution of silk fibroin and preparing an aqueous solution of gelatine in volume ratio of 7:3 and 0.8–1.2 vol. % dimethylsulphoxide (DMSO) with subsequent defrosting and treatment with 96 % ethanol, which provides formation β-flat structure, then scaffolds are frozen in distilled water. Creeping of scaffold obtained at the stage of composite scaffold formation is carried out in 70 % ethanol using a dispersant, followed by sorting obtained scaffold fragments to obtain composite microparticles with size of 100–250 mcm. Deposition of composite microparticles obtained at cryofilling of scaffold is suspended from suspension in 70
