A culture bottle structure improvement (1) is mainly composed of a bottle cap and a bottle body; the bottle cap is provided with an internal thread section, and the bottle body is provided with an external thread section, and the bottle cap is fixed by the internal thread section and the external thread section of the bottle body; the characteristics of the improvement are: the bottle body is provided with a rubber washer under the external thread section to install the rubber washer, and the gas closure of the bottle cap and the bottle body can be avoided Pollutants enter the bottle body to contaminate the medium.一種培養瓶結構改良(一),主要具有一瓶蓋及一瓶身所構成;該瓶蓋設置有內螺紋段,該瓶身設置有外螺紋段,該瓶蓋藉由內螺紋段與瓶身的外螺紋段螺鎖固定一體;其特徵在於:該瓶身在外螺紋段下方設置一橡膠墊圈安裝其中,而得到瓶蓋與瓶身氣閉合,可避免污染物進入瓶身內汙染培養基。