Paulus Jacobus Antonius SONDERMEIJER,Lisette SANDERS,Karin Huberdina Antonia VAN DER HEIJDEN-LIEFKENS
申请号：
US16756490
公开号：
US20200299726A1
申请日:
2018.10.16
申请国别(地区):
US
年份:
2020
代理人:
摘要：
The present invention relates to the field of veterinary vaccines, in particular to porcine vaccines against PCV2 and associated diseases. Specifically the invention relates to the finding that a mutation is required in PCV2b ORF2 protein, to prevent its nuclear accumulation upon expression in insect cells； the mutation introduces a Proline at amino acid position 131. This allows efficient expression in insect cells, easy harvesting, and generates large amounts of virus-like particles. The VLPs are highly effective in vaccines for porcines for reduction of infection by PCV2 or of associated signs of disease.
