Disclosed is a nutrient solution disinfection system, comprising: at least one cultivation system (102) comprising a cultivation device (104) for growing plants and a disinfection sub-device (108) for cyclic disinfection inside the cultivation device; an overall processing system (202) comprising an overall disinfection device (206) and used for disinfecting a nutrient solution from each cultivation system; and a micro-controller (302) connected to the overall processing system and each cultivation system. Further disclosed is a culture device including the nutrient solution disinfection system. The nutrient solution disinfection system and the culture device effectively control the ingredients and microorganism contents of the nutrient solution, improve culturing effects and reduce the influence of bacteria on plant growth.