An isolated antibody, consisting of an anti-glutathionylated eNOS antibody, wherein the anti-glutathionylated eNOS antibody has been generated against an immunogen consisting of a peptide that includes glutathione； a first linker； an eNOS peptide； a second linker； and a T-cell epitope； and wherein the anti-glutathionylated eNOS antibody is adapted to recognize redox modulated eNOS proteins.
