In an unextracted tooth root canal filler, dental tissues can be regenerated in a tooth with a complete apical closure, with none of internal resorption and external resorption and no odontoclasts being observed and odontoblasts being lined along the dentinal wall. An unextracted tooth root canal filler 200 includes a dental pulp stem cells 220 and an extracellular matrix 210, and is formed to be inserted in an apical part of a root canal of an unextracted tooth after pulpectomy or after enlargement and cleaning of the infected root canal. The dental pulp stem cells are dental pulp CXCR4-positive cells, for example. A chemotactic factor including at least one of a cell chemotactic factor, a cell growth factor, a neurotrophic factor, or an angiogenic factor is preferably transplanted to the tooth crown part of the root canal.
