BIOINOVA, S.R.O.;INSTITUTE OF EXPERIMENTAL MEDICINE AS CR; V.V.I.;INSTITUTE OF EXPERIMENTAL MEDICINE AS CR, V.V.I.
发明人:
PETRENKO, Yuriy,SYKOVA, Eva,CEJKOVA, Jitka,VACKOVA, Irena,GROH, Tomas
申请号：
IBIB2017/052832
公开号：
WO2017/195179A1
申请日:
2017.05.13
申请国别(地区):
IB
年份:
2017
代理人:
摘要：
A solution for the preservation, transport and application of stem cells for medical utilisation, that consists ofa buffer water solution of trehalose. The solution contains cations K+ in a quantity of 20 to 50 mmol/1, cations Na+ in a quantity of 20 to 80 mmol/1； anions CI" in a quantity of 0.5 to 40 mmol/1, anions PO43" and/or HPO42" and/or H2PO4" in a total quantity of 20 to 65 mmol/1, and trehalose in a quantity of 60 to 300 mmol/1 as necessary components. The concentration of the components has been selected in such a way that the pH of the solution is 7.1 to 7.6. The solution is intended for storing the cells at a temperature of - 4 °C to 25 °C, advantageously 0 °C to 8 °C, and most frequently 4 °C to 6 °C. Adequate viability of the cells is kept for a period of at least 72 hours.. The solution consists of pharmaceutically acceptable compounds, and it can be used for application of the stem cells that are kept directly within. The solution containing the stem cells is intended for the treatment of inflammatory diseases including post-traumatic inflammatory reactions after damage or injury, as well as for treatment of degenerative and neurodegenerative diseases. The solution containing the stem cells can also be used for the treatment of trauma, of developmental defects, for healing of wounds and skin burns, for tissue replacement, for treatment of locomotion system diseases (tendons, joints, inflammatory diseases, arthritis, and osteoarthritis), bone defects, diabetes, attacks, and cardiological and oncological diseases.L'invention concerne une solution, pour la conservation, le transport et l'application de cellules souches à un usage médical, qui est constituée d'une solution tampon aqueuse de tréhalose. La solution contient des cations K+ en une quantité de 20 à 50 mmol/1, des cations Na+ en une quantité de 20 à 80 mmol/1 ； des anions CI" en une quantité de 0,5 à 40 mmol/1, des anions PO43" et/ou HPO42" et/ou H2PO4" en une quantité totale de 20 à 65 mmol/1,
