The invention relates to a pump chamber (1) for a peristaltic pump, the pump chamber (1) comprising an elongate cavity (1.1) defined between an elastically deformable chamber wall (1.2) and a rigid chamber (1.3) wall, wherein the elastically deformable wall (1.2) and the rigid wall (1.3) are arranged as a one-piece part by two-component injection molding. The invention also refers to a method for producing the pump chamber (1) for a peristaltic pump, the method comprising a two-component injection molding of the elastically deformable wall (1.2) and the rigid chamber wall (1.3) thereby forming them as a one-piece part.