DRAGHIA-AKLI, RUXANDRA,CARPENTER, ROBERT H.,KERN, DOUGLAS R.,SCHWARTZ, ROBERT J.,KING, GLEN,HAHN, KEVIN,BRENNER, MALCOLM K.
申请号：
CA2469310
公开号：
CA2469310C
申请日:
2002.12.10
申请国别(地区):
CA
年份:
2013
代理人:
摘要：
The present invention pertains to compositions and methods for plasmid-mediated supplementation. The compositions and method are useful for retardingthe growth of the tumor, and retarding cachexia, wasting, anemia and othereffects that are commonly associated in cancer bearing animals. Overall, theembodiments of the invention can be accomplished by delivering an effectiveamount of a nucleic acid expression construct that encodes a GHRH orfunctional biological equivalent thereof into a tissue of an animal andallowing expression of the encoded gene in the animal. For example, when sucha nucleic acid sequence is delivered into the specific cells of the animaltissue specific constitutive expression is achieved. Furthermore, externalregulation of the GHRH or functional biological equivalent thereof gene can beaccomplished by utilizing inducible promoters that are regulated by molecularswitch molecules, which are given to the animal. The preferred method todeliver the constitutive or inducible nucleic acid encoding sequences of GHRHor the functional biological equivalents thereof is directly into the cells ofthe animal by the process of in vivo electroporation. In addition, a treatmentfor retarding the growth of the tumor, and retarding cachexia or the wastingeffects that are commonly associated with tumors is achieved by the deliveryof recombinant GHRH or biological equivalent into the animal.
