A method of obtaining a fluorescent fundus image having following steps is provided, the steps include: receiving a to-be-detected color fundus image; obtaining a color feature of the to- be-detected color fundus image; encoding the color feature of the to- be-detected color fundus image according to a fundus image transformation model to transform the to-be-detected color fundus image into a to-be-detected fluorescent fundus image; and outputting the to-be-detected fluorescent fundus image.本發明的用以獲取螢光眼底圖的方法具有下列步驟:接收待測彩色眼底圖;獲取待測彩色眼底圖的彩圖特徵;依據眼底影像圖轉換模型編碼待測彩色眼底圖的彩圖特徵,以轉換待測彩色眼底圖為待測螢光眼底圖;以及輸出螢光眼底圖。